Regulation of microbial populations by coral surface mucus and mucus-associated bacteria
نویسندگان
چکیده
منابع مشابه
New method for counting bacteria associated with coral mucus.
The ability to count bacteria associated with reef-building corals in a rapid, reliable, and cost-effective manner has been hindered by the viscous and highly autofluorescent nature of the coral mucus layer (CML) in which they live. We present a new method that disperses bacterial cells by trypsinization prior to 4',6-diamidino-2-phenylindole (DAPI) staining and quantification by epifluorescenc...
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In the oligotrophic marine environment there are ecological niches rich in nutrients and diverse in bacterial populations. One such niche is the coral surface mucus layer. Interactions amongst microorganisms found in coral mucus may be symbiotic or competitive; competing over space and food. It has been hypothesized that the microbial communities found on the coral surface may play a role in th...
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The distribution of viruses inhabiting the coral mucus remains undetermined, as there is no suitable standardized procedure for their separation from this organic matrix, principally owing to its viscosity and autofluorescence. Seven protocols were tested, and the most efficient separations were obtained from a chemical treatment requiring potassium citrate.
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The relative abundance of bacteria in the mucus and crushed tissue of the Mediterranean coral Oculina patagonica was determined by analyses of the 16S rRNA genes of isolated colonies and from a 16S rRNA clone library of extracted DNA. By SYBR gold staining, the numbers of bacteria in mucus and tissue samples were 6.2 x 10(7) and 8.3 x 10(8)/cm2 of coral surface, respectively, 99.8% of which fai...
متن کاملCounting viruses in coral mucus
3 4 5 6 Amandine Leruste, Thierry Bouvier, Yvan Bettarel 7 8 9 10 UMR 5119 ECOSYM – Université Montpellier 2IRDIFREMER CNRS (France) 11 12 13 Running head : Mucus-associated viruses 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 *Corresponding author : [email protected] 42 43 IRD-UMR 5119 Ecologie des Systèmes Marins Côtiers (ECOSYM) 44 Université de Mont...
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ژورنال
عنوان ژورنال: Marine Ecology Progress Series
سال: 2006
ISSN: 0171-8630,1616-1599
DOI: 10.3354/meps322001